From UV trace to fraction pool.

Analyse FPLC and HPLC traces, plan fraction pools, and learn the science behind your separation.

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Illustrative size-exclusion chromatogramUV absorbance against elution volume with a small early peak, a shoulder, a main peak near 12 mL and later material. Half-millilitre fractions run along the volume axis; fractions A12 to B3 are shaded as a candidate pool.Pool A12–B307501500A280 (mAU)A1A5A9A12B3B5B9C148121620Elution volume (mL)
Illustrative SEC trace · shaded fractions show a candidate pool. Not measured data.

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