Chromatography Academy
Free, practical material for researchers running FPLC and HPLC, from first principles to operating an ÄKTA™.
Tutorials
Step-by-step bench workflows, from a breakthrough run to measured dynamic binding capacity.
Fundamentals
Plate theory, resolution and selectivity: the concepts behind every separation.
Techniques
Size exclusion, ion exchange, hydrophobic interaction, affinity and reversed phase.
Method development
Plan a purification: set the goal, order the steps, scout buffers and load, and scale up.
ÄKTA™ and UNICORN™
System hardware, the method editor, and evaluating runs in UNICORN™.
Data analysis
Import a chromatogram, choose a baseline, integrate an interval, and keep the evidence.
Troubleshooting
Tailing peaks, poor resolution, ghost peaks and baseline drift.
Glossary
Dead volume, theoretical plates, and every term in between.
