Tools
Calculators for everyday column work. Each one shows its formulas and assumptions, and nothing you enter is saved.
Flow rate, linear velocity and residence time
Convert mL/min, cm/h, residence time and CV/h for a column of given inner diameter and bed height.
Buffer pH at run temperature
How far a buffer’s pH moves between bench and cold room, from evaluated ionization enthalpies for ten common buffers.
Salt gradient planner
Slope in mM per CV, volumes, times and buffer use for wash, gradient and strip, with system dwell volume.
Scale-up at constant residence time(in tutorial)
Flow, load and gradient volume for a larger column, with the same or a different bed height.
Dynamic binding capacity(in tutorial)
DBC from feed concentration, breakthrough volume, dead volume and packed column volume.